Isolation and characterization of 13 polymorphic nuclear microsatellite primers for the widespread Indo-Pacific three-spot damselfish, Dascyllus trimaculatus, and closely related D. auripinnis

Isolation and characterization of 13 polymorphic nuclear microsatellite primers for the widespread Indo-Pacific three-spot damselfish, Dascyllus trimaculatus, and closely related D. auripinnis

Year: 

2009
Authors: 
Leray, M.Beldade, R.Holbrook, S. J.Schmitt, R. J.Planes, S.Bernardi, G.

Source: 

Molecular Ecology Resources

Abstract: 

A set of 13 simple sequence repeat markers was developed from D. trimaculatus genomic DNA, tested for D. auripinnis and characterized using 40 individuals per species. All the loci were polymorphic with a number of alleles ranging from three to 30. Observed heterozygosities varied from 0.23 to 0.89 for D. trimaculatus and from 0.11 to 0.85 for D. auripinnis. Early results show that these will be powerful markers for the study of ecological and evolutionary mechanism in this coral reef fish species complex.

Volume: 

9

Issue: 

1

Pages: 

213-215

ISBN: 

1755-098X

Publication Type: 

Journal Article

ISI Number: 

ISI:000262678900047

Research Areas: